RSS

PDB:2A65

Protein Name

Bacterial homolog of Na+/Cl- dependent neurotransmitter transporters

Species

Aquifex aeolicus (thermophile, bacteria) VF5

Biological Context

Neurons in the brain rapidly communicate with each other in the following manner:

  1. Electrical signals are transmitted to the presynaptic terminal of the first neuron.
  2. The neurotransmitters in synaptic vesicle are released to the extracellular space (synaptic cleft).
  3. The released neurotransmitters bind to the transmembrane receptor protein (neurotransmitter-gated ion channels).
  4. The gate opens and the electric potential of the membrane changes.

The rapid communication requires not only to release neurotransmitters rapidly to synaptic clefts but also to re-uptake unnecessary neurotransmitters rapidly. This procedure removes excess neurotransmitters and prepares for next signals. The re-uptake of neurotransmitters is performed by neurotransmitter sodium symporters (NSSs) and the up-taken neurotransmitters are later reused. This thermodynamically uphill transports use sodium and chloride electrochemical gradients. Substances to transport are wide, and lack of Na+/Cl--dependent transporters function causes multiple disorders, such as depression, Parkinson's disease, orthostatic intolerance and epilepsy.

Structure Description

2a652a65_x2a65_y

The structure shown here is a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters (LeuTAa) from Aquifex aeolicus complexed with a substrate (leucine), two sodium ions, a chloride ion. It consists of 12 transmembrane alpha helices, and TM1-TM5 and TM6-TM10 form similar structures. The leucine are bound to the protein core, which exists in halfway across the membrane, partially unwound helices, with hydrogen bonds. Sodium ions also exist near the core, stabilizing the ligand binding site.

Although this structure is the closed state including ligands, it is also indicative of the system of re-take which open the outer side and inner side alternately.

Protein Data Bank (PDB)

References

Source

  • Yamashita, A. Singh, S.K. Kawate, T. Jin, Y. Gouaux, E.; "Crystal structure of a bacterial homologue of Na(+)/Cl(-)-dependent neurotransmitter transporters."; Nature; (2005) 437:215-223 PubMed:16041361.

Others

author: Sachiyo Nomura, translated by Takahiro Kudou


Japanese version:PDB:2A65